Welcome again, guys. Uh, we are talking about / in this video / about / overview of how / know / about / purpose / in lab. Now / cell / actually, uh, is a multi … uh … multi-stage process / four / major / now, let’s begin with it / first / do here is … uh … / taking up all / in / preservation / take / population / and isolate … uh … from the tissue / thing is / in this case / taught / process / already going on / lab / already going on / so, you will get the cell / already been isolated from the tissue / preserved / process / Now, the thing is, if you want to collect / process / become much more complicated / scrap of tissue / from / individual of your interest / process / reduce / debris / particles / outside / other materials / culture media / generation / then you take / cell / preservation / material / simply revive those / of your interest / your hand / second stage is, maintaining / culture / culture / culture / liquid media / called / suspension culture / you can grow / solid surface / which is most common of all / solid / grow / cover / surface / you continue … uh … to grow those cells / during / follow / technique / throughout the time / if / any time / contaminated / experiment will be ruined. Right? / now / means / two / one / need / culture / once / surface / kind of, uh, covered with the cells / old culture / into __ new culture / fresh culture media / process / sub / consist__ of / subsequently / until / unless / you are satisfied / done / experiment / now / after / told / that means … uh … once / take / from / media / fresh / the cell / sub / after the subculture / is done / store / storing of / is very very important / someone else / same type of cell / experiment / chemical properties / properties / for that purpose, you need to store / right / for storing / require / process of cryopreservation / before / count / exact number of cells / storing / density / store them / preservation / future / find out / we will be talking / each / different steps / in a little bit more detail / how / done / Thank you.
