In biology, cell culture is the process __ __ cells / grown / controlled conditions outside of its native environment / scientists to use cells, the fundamental unit of life / understanding / physiological processes in the human body. One of the major contributions __ cell culturing / the development of p__ v__ / back in / researchers had to use / live animals to grow p__ __ / cell culture / researchers __ able to create / which eventually led to the creation of the v__. / to create properly __ed / p__s, such as __ __ antibodies / make / e__ cells / because e__ cells __ __ modifications. Nowadays, cell culture is being used widely / particularly in the field / v__ research, cancer research and p__ t__.
Cell __ used in laboratories can be generalized into two categories: primary cells and established cell lines / are cells that / directly prepared from an organism’s tissues / under the right conditions / primary / grow / p__ / only able to do so a finite number of times. This is because / each / divide / is lost / after a certain number of cell divisions, the / cells can no longer divide. / Established cell lines, on the other hand, are able to escape / normal / cell / grow indefinitely / extremely useful for __ long-term research
Whereas primary cells / obtained directly / d__ tissues / derive / created from transforming / chemical treatments / or treatments, target one of the checkpoints / five phases / m__ cell cycle. Checkpoints / there / to ensure / meet / appropriate requirement / in order to / daughter cells. One / at the end / which allow / cell division / the second / check the quality / of the replicated DNA and / determine / any / need to __ __ The third checkpoint / purposes / again, to ensure / replicated completely and is undamaged. / Finally, there are several checkpoints within m__ / ensure / proper position / to complete cell division. / it is because / evade / cell cycle / allowed to / continue / many v__ o__ / such as / in human / cell cycle / namely, the t__ __ __ normal / cancers.
primary cells / established cell line / culture / usually / two / forms / or free-floating in culture media / are also often referred to / are called suspension cultures. Knowing the growth properties of the cells / choose / for their experiments. To keep the cells alive / conditions / conditions as possible. Therefore, / organic / vitamins are added into the culture media / Traditionally, other / factors / / bovine / However, there are a lot of uncertainties with regards to / For example, / know / exact composition / and this, in turn, / effect / exact composition / known / address this issue.
As / ph__ fluids / neutral / culture / to ensure / proper / maintained / one / to keep / pH / carbon dioxide / another common buffering / involves / such as __. In both kinds, / common / indicator / added to the media / visually / change in pH. / Different cell lines generally require / different / commercial / or / __ __ M__ institute / 1640 / mixer / apart from using / appropriate media for for growth / also maintained / correct temperature / For example, most m__ / incubated / optimal / cold-blooded animals / should be kept at a lower temperature. Other measures to take / , such as bacteria, f__ or m__, can ruin / invalid experimental data / always used / clean / air / outside contaminants / from entering the culture.
In addition / contaminated / other fast-growing / over / replaced by other / introduced / The consequence / making / scientific conclusions / cell / profiling, or / is recommended __ __ from time to time. /
provides / products / such as / media and plastic-ware. We also have specialized / drug / m__ contamination / Lastly, / agencies / authentication / profiling / we are proud to / offer / service / our customers. Cell culture technique / advanced / since / provide us valuable information / all / biology. If / please visit / knowledge base / sure / next / cell / techniques / we will answer them as soon as possible.
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